Method for simultaneous measurements of traces of heptadeuterated cholesterol and cholesterol by gas chromatography–mass spectrometry: application in humans
Identifieur interne : 002E75 ( Main/Exploration ); précédent : 002E74; suivant : 002E76Method for simultaneous measurements of traces of heptadeuterated cholesterol and cholesterol by gas chromatography–mass spectrometry: application in humans
Auteurs : Gabrielle Beaumier-Gallon [France] ; Jacques Lanfranchi [France] ; Marie-France Vergnes [France] ; Denis Lairon [France] ; Jean Pastor [France] ; Anne Marie Pauli [France] ; Henri Portugal [France]Source :
- Journal of Chromatography B: Biomedical Sciences and Applications [ 0378-4347 ] ; 1998.
Abstract
An assay was developed to quantify deuterated cholesterol (used as a tracer) and cholesterol using gas chromatography–mass spectrometry. Ergosterol and epicoprostanol were used as internal standards. Deuterated cholesterol was quantified by comparing its peak area to that of epicoprostanol and cholesterol to ergosterol. The mean absolute recovery in spiked serum was 99.96%; the precision was in the range 0.16–10.9% and accuracy 90.4–100%; the limit of detection in plasma was 3·10−5 mmol l−1. Using two internal standards, the method described herein seems particularly suitable for application in humans i.e., measuring traces of deuterated cholesterol (range: 0–6.26·10−4 mmol l−1) along with natural cholesterol (range: 0.065–4.42 mmol l−1) in human plasma and lipid fractions postprandially.
Url:
DOI: 10.1016/S0378-4347(98)00364-8
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000C49
- to stream Istex, to step Curation: 000A22
- to stream Istex, to step Checkpoint: 002665
- to stream Main, to step Merge: 002F58
- to stream Main, to step Curation: 002E75
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Method for simultaneous measurements of traces of heptadeuterated cholesterol and cholesterol by gas chromatography–mass spectrometry: application in humans</title><author><name sortKey="Beaumier Gallon, Gabrielle" sort="Beaumier Gallon, Gabrielle" uniqKey="Beaumier Gallon G" first="Gabrielle" last="Beaumier-Gallon">Gabrielle Beaumier-Gallon</name></author><author><name sortKey="Lanfranchi, Jacques" sort="Lanfranchi, Jacques" uniqKey="Lanfranchi J" first="Jacques" last="Lanfranchi">Jacques Lanfranchi</name></author><author><name sortKey="Vergnes, Marie France" sort="Vergnes, Marie France" uniqKey="Vergnes M" first="Marie-France" last="Vergnes">Marie-France Vergnes</name></author><author><name sortKey="Lairon, Denis" sort="Lairon, Denis" uniqKey="Lairon D" first="Denis" last="Lairon">Denis Lairon</name></author><author><name sortKey="Pastor, Jean" sort="Pastor, Jean" uniqKey="Pastor J" first="Jean" last="Pastor">Jean Pastor</name></author><author><name sortKey="Pauli, Anne Marie" sort="Pauli, Anne Marie" uniqKey="Pauli A" first="Anne Marie" last="Pauli">Anne Marie Pauli</name></author><author><name sortKey="Portugal, Henri" sort="Portugal, Henri" uniqKey="Portugal H" first="Henri" last="Portugal">Henri Portugal</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:0393C46727E4DB38C68004D1362FE58D86F9E9B0</idno><date when="1998" year="1998">1998</date><idno type="doi">10.1016/S0378-4347(98)00364-8</idno><idno type="url">https://api.istex.fr/document/0393C46727E4DB38C68004D1362FE58D86F9E9B0/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000C49</idno><idno type="wicri:Area/Istex/Curation">000A22</idno><idno type="wicri:Area/Istex/Checkpoint">002665</idno><idno type="wicri:doubleKey">0378-4347:1998:Beaumier Gallon G:method:for:simultaneous</idno><idno type="wicri:Area/Main/Merge">002F58</idno><idno type="wicri:Area/Main/Curation">002E75</idno><idno type="wicri:Area/Main/Exploration">002E75</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Method for simultaneous measurements of traces of heptadeuterated cholesterol and cholesterol by gas chromatography–mass spectrometry: application in humans</title><author><name sortKey="Beaumier Gallon, Gabrielle" sort="Beaumier Gallon, Gabrielle" uniqKey="Beaumier Gallon G" first="Gabrielle" last="Beaumier-Gallon">Gabrielle Beaumier-Gallon</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Unité-476 INSERM, 18 Avenue Mozart, 13009 Marseille</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Lanfranchi, Jacques" sort="Lanfranchi, Jacques" uniqKey="Lanfranchi J" first="Jacques" last="Lanfranchi">Jacques Lanfranchi</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Chimie Analytique, Faculté de Pharmacie, Avenue Jean Moulin, 13385 Marseille Cedex 5</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Vergnes, Marie France" sort="Vergnes, Marie France" uniqKey="Vergnes M" first="Marie-France" last="Vergnes">Marie-France Vergnes</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Chimie Analytique, Faculté de Pharmacie, Avenue Jean Moulin, 13385 Marseille Cedex 5</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Lairon, Denis" sort="Lairon, Denis" uniqKey="Lairon D" first="Denis" last="Lairon">Denis Lairon</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Unité-476 INSERM, 18 Avenue Mozart, 13009 Marseille</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Pastor, Jean" sort="Pastor, Jean" uniqKey="Pastor J" first="Jean" last="Pastor">Jean Pastor</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Chimie Analytique, Faculté de Pharmacie, Avenue Jean Moulin, 13385 Marseille Cedex 5</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Pauli, Anne Marie" sort="Pauli, Anne Marie" uniqKey="Pauli A" first="Anne Marie" last="Pauli">Anne Marie Pauli</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Chimie Analytique, Faculté de Pharmacie, Avenue Jean Moulin, 13385 Marseille Cedex 5</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Portugal, Henri" sort="Portugal, Henri" uniqKey="Portugal H" first="Henri" last="Portugal">Henri Portugal</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Chimie Analytique, Faculté de Pharmacie, Avenue Jean Moulin, 13385 Marseille Cedex 5</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation><affiliation><wicri:noCountry code="no comma">Corresponding author.</wicri:noCountry></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Chromatography B: Biomedical Sciences and Applications</title><title level="j" type="abbrev">CHROLD</title><idno type="ISSN">0378-4347</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998">1998</date><biblScope unit="volume">718</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="23">23</biblScope><biblScope unit="page" to="32">32</biblScope></imprint><idno type="ISSN">0378-4347</idno></series><idno type="istex">0393C46727E4DB38C68004D1362FE58D86F9E9B0</idno><idno type="DOI">10.1016/S0378-4347(98)00364-8</idno><idno type="PII">S0378-4347(98)00364-8</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0378-4347</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">An assay was developed to quantify deuterated cholesterol (used as a tracer) and cholesterol using gas chromatography–mass spectrometry. Ergosterol and epicoprostanol were used as internal standards. Deuterated cholesterol was quantified by comparing its peak area to that of epicoprostanol and cholesterol to ergosterol. The mean absolute recovery in spiked serum was 99.96%; the precision was in the range 0.16–10.9% and accuracy 90.4–100%; the limit of detection in plasma was 3·10−5 mmol l−1. Using two internal standards, the method described herein seems particularly suitable for application in humans i.e., measuring traces of deuterated cholesterol (range: 0–6.26·10−4 mmol l−1) along with natural cholesterol (range: 0.065–4.42 mmol l−1) in human plasma and lipid fractions postprandially.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Provence-Alpes-Côte d'Azur</li></region><settlement><li>Marseille</li></settlement></list><tree><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Beaumier Gallon, Gabrielle" sort="Beaumier Gallon, Gabrielle" uniqKey="Beaumier Gallon G" first="Gabrielle" last="Beaumier-Gallon">Gabrielle Beaumier-Gallon</name></region><name sortKey="Lairon, Denis" sort="Lairon, Denis" uniqKey="Lairon D" first="Denis" last="Lairon">Denis Lairon</name><name sortKey="Lanfranchi, Jacques" sort="Lanfranchi, Jacques" uniqKey="Lanfranchi J" first="Jacques" last="Lanfranchi">Jacques Lanfranchi</name><name sortKey="Pastor, Jean" sort="Pastor, Jean" uniqKey="Pastor J" first="Jean" last="Pastor">Jean Pastor</name><name sortKey="Pauli, Anne Marie" sort="Pauli, Anne Marie" uniqKey="Pauli A" first="Anne Marie" last="Pauli">Anne Marie Pauli</name><name sortKey="Portugal, Henri" sort="Portugal, Henri" uniqKey="Portugal H" first="Henri" last="Portugal">Henri Portugal</name><name sortKey="Vergnes, Marie France" sort="Vergnes, Marie France" uniqKey="Vergnes M" first="Marie-France" last="Vergnes">Marie-France Vergnes</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Musique/explor/MozartV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002E75 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002E75 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Musique
|area= MozartV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:0393C46727E4DB38C68004D1362FE58D86F9E9B0
|texte= Method for simultaneous measurements of traces of heptadeuterated cholesterol and cholesterol by gas chromatography–mass spectrometry: application in humans
}}
| This area was generated with Dilib version V0.6.20. |  |